The Route 66 Challenge event has been run every two years since 2002 and takes in 4,000km of the old route, raising money for Temple Street Childrens Hospital.
Past Challenges have raised a combined €3m which has been used in the refurbishment of the Accident/Emergency Unit, the Renal Unit, the purchase of a new CT Scanner and the building of a dedicated isolation unit for Cystic Fibrosis.
The participants this year hope to raise a further €300,000.
